Pastalkova, E. et al. Storage of spatial information of LTP by the maintenance mechanism. Science 313, 1141–1144 (2006).
Google Scholar
Lee, A. M. et al. Prkcz null mice show normal learning and memory. Nature 493, 416–419 (2013).
Google Scholar
Volk, L. J., Bachman, J. L., Johnson, R., Yu, Y. & Huganir, R. L. PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory. Nature 493, 420–423 (2013).
Google Scholar
Kelly, M. T., Crary, J. F. & Sacktor, T. C. Regulation of protein kinase Mζ synthesis by multiple kinases in long-term potentiation. J. Neurosci. 27, 3439–3444 (2007).
Google Scholar
Gao, P. P., Goodman, J. H., Sacktor, T. C. & Francis, J. T. Persistent increases of PKMζ in sensorimotor cortex maintain procedural long-term memory storage. iScience 5, 90–98 (2018).
Google Scholar
Tsokas, P. et al. Compensation for PKMζ in long-term potentiation and spatial long-term memory in mutant mice. eLife 5, e14846 (2016).
Google Scholar
Lin, J. & Alexander-Katz, A. Cell membranes open “doors” for cationic nanoparticles/biomolecules: insights into uptake kinetics. ACS Nano 7, 10799–10808 (2013).
Google Scholar
Felgner, P. L. et al. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl Acad. Sci. USA 84, 7413–7417 (1987).
Google Scholar
Kaplan, I. M., Wadia, J. S. & Dowdy, S. F. Cationic TAT peptide transduction domain enters cells by macropinocytosis. J. Control. Release 102, 247–253 (2005).
Google Scholar
Sacktor, T. C. & Fenton, A. A. Appropriate application of ZIP for PKMζ inhibition, LTP reversal, and memory erasure. Hippocampus 22, 645–647 (2012).
Google Scholar
Thompson, D. B., Villaseñor, R., Dorr, B. M., Zerial, M. & Liu, D. R. Cellular uptake mechanisms and endosomal trafficking of supercharged proteins. Chem. Biol. 19, 831–843 (2012).
Google Scholar
Green, M. & Loewenstein, P. M. Autonomous functional domains of chemically synthesized human immunodeficiency virus tat trans-activator protein. Cell 55, 1179–1188 (1988).
Google Scholar
Frankel, A. D. & Pabo, C. O. Cellular uptake of the tat protein from human immunodeficiency virus. Cell 55, 1189–1193 (1988).
Google Scholar
Sadeh, N., Verbitsky, S., Dudai, Y. & Segal, M. Zeta inhibitory peptide, a candidate inhibitor of protein kinase M, is excitotoxic to cultured hippocampal neurons. J. Neurosci. 35, 12404–12411 (2015).
Google Scholar
Serrano, P. et al. PKMζ maintains spatial, instrumental, and classically conditioned long-term memories. PLoS Biol. 6, 2698–2706 (2008).
Google Scholar
Kopec, C. D., Li, B., Wei, W., Boehm, J. & Malinow, R. Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. J. Neurosci. 26, 2000–2009 (2006).
Google Scholar
Miesenbock, G., De Angelis, D. A. & Rothman, J. E. Visualizing secretionand synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192–195 (1998).
Google Scholar
Bingor, A., Haham, T., Thornton, C., Stern-Bach, Y. & Yaka, R. Zeta inhibitory peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors. Nat. Commun. 11, 3688 (2020).
Google Scholar
Kawasaki-Nishi, S., Bowers, K., Nishi, T., Forgac, M. & Stevens, T. H. The amino-terminal domain of the vacuolar proton-translocating ATPase A subunit controls targeting and in vivo dissociation, and the carboxyl-terminal domain affects coupling of proton transport and ATP hydrolysis. J. Biol. Chem. 276, 47411–47420 (2001).
Google Scholar
Banker, G. A. & Cowan, W. M. Rat hippocampal neurons in dispersed cell culture. Brain Res. 126, 397–425 (1977).
Google Scholar
Turrigiano, G. G., Leslie, K. R., Desai, N. S., Rutherford, L. C. & Nelson, S. B. Activity-dependent scaling of quantal amplitude in neocortical neurons. Nature 391, 892–896 (1998).
Google Scholar
Otmakhov, N. et al. Forskolin-induced LTP in the CA1 hippocampal region is NMDA receptor dependent. J. Neurophysiol. 91, 1955–1962 (2004).
Google Scholar
Serrano, P., Yao, Y. & Sacktor, T. C. Persistent phosphorylation by protein kinase Mζ maintains late-phase long-term potentiation. J. Neurosci. 25, 1979–1984 (2005).
Google Scholar
Crosby, K. C. et al. Nanoscale subsynaptic domains underlie the organization of the inhibitory synapse. Cell Rep. 26, 3284–3297.e3 (2019).
Google Scholar
Swanson, J. A. & Watts, C. Macropinocytosis. Trends Cell Biol. 5, 424–428 (1995).
Google Scholar
Casamento, A. & Boucrot, E. Molecular mechanism of fast endophilin-mediated endocytosis. Biochem. J. 477, 2327–2345 (2020).
Do-Monte, F. H., Quinõnes-Laracuente, K. & Quirk, G. J. A temporal shift in the circuits mediating retrieval of fear memory. Nature 519, 460–463 (2015).
Google Scholar
Corcoran, K. A. & Quirk, G. J. Activity in prelimbic cortex is necessary for the expression of learned, but not innate, fears. J. Neurosci. 27, 840–844 (2007).
Google Scholar
Sacco, T. & Sacchetti, B. Role of secondary sensory cortices in emotional memory storage and retrieval in rats. Science 329, 649–656 (2010).
Kourrich, S., Rothwell, P. E., Klug, J. R. & Thomas, M. J. Cocaine experience controls bidirectional synaptic plasticity in the nucleus accumbens. J. Neurosci. 27, 7921–7928 (2007).
Google Scholar
Li, Y. et al. Inhibition of PKMz in nucleus accumbens core abolishes long-term drug reward memory. J. Neurosci. 31, 5436–5446 (2011).
Google Scholar
Dumas, T. C. Late postnatal maturation of excitatory synaptic transmission permits adult-like expression of hippocampal-dependent behaviors. Hippocampus 15, 562–578 (2005).
Google Scholar
Vives, E., Richard, J.-, Rispal, C. & Lebleu, B. TAT peptide internalization: seeking the mechanism of entry. Curr. Protein Pept. Sci. 4, 125–132 (2005).
Google Scholar
Sloley, S. S. et al. High-frequency head impact causes chronic synaptic adaptation and long-term cognitive impairment in mice. Nat. Commun. 12, 2613 (2021).
Google Scholar
Tye, K. M., Stuber, G. D., De Ridder, B., Bonci, A. & Janak, P. H. Rapid strengthening of thalamo-amygdala synapses mediates cue-reward learning. Nature 453, 1253–1257 (2008).
Google Scholar
Everitt, B., Morris, K., O’Brien, A. & Robbins, T. The basolateral amygdala-ventral striatal system and conditioned place preference: further evidence of limbic-striatal interactions underlying reward-related processes. Neuroscience 42, 1–18 (1991).
Google Scholar
Nabavi, S. et al. Engineering a memory with LTD and LTP. Nature 511, 348–352 (2014).
Google Scholar
Stevens, C. F. A million dollar question: minireview does LTP = memory? Neuron 20, 1–2 (1998).
Google Scholar
Compans, B., Choquet, D. & Hosy, E. Review on the role of AMPA receptor nano-organization and dynamic in the properties of synaptic transmission. Neurophotonics 3, 041811 (2016).
Google Scholar
Hou, Q., Zhang, D., Jarzylo, L., Huganir, R. L. & Man, H.-Y. Homeostatic regulation of AMPA receptor expression at single hippocampal synapses. Proc. Natl Acad. Sci. USA 105, 775–780 (2008).
Sutton, G. & Chandler, L. J. Activity-dependent NMDA receptor-mediated activation of protein kinase B/Akt in cortical neuronal cultures. J. Neurochem. 82, 1097–1105 (2002).
Google Scholar
Boucrot, E. et al. Endophilin marks and controls a clathrin-independent endocytic pathway. Nature 517, 460–465 (2015).
Google Scholar
Triller, A. & Choquet, D. New concepts in synaptic biology derived from single-molecule imaging. Neuron 59, 359–374 (2008).
Google Scholar
Choquet, D. & Triller, A. The dynamic synapse. Neuron 80, 691–703 (2013).
Google Scholar
Tatavarty, V., Sun, Q. & Turrigiano, G. G. How to scale down postsynaptic strength. J. Neurosci. 33, 13179–13189 (2013).
Google Scholar
Dong, Z. et al. Long-term potentiation decay and memory loss are mediated by AMPAR endocytosis. J. Clin. Invest. 125, 234–247 (2015).
Google Scholar
Ge, Y. et al. Hippocampal long-term depression is required for the consolidation of spatial memory. Proc. Natl Acad. Sci. USA 107, 16697–16702 (2010).
Google Scholar
Brebner, K. et al. Nucleus accumbens long-term depression and the expression of behavioral sensitization. Science 310, 1337–1340 (2005).
Google Scholar
Buard, I. et al. CaMKII “autonomy” is required for initiating but not for maintaining neuronal long-term information storage. J. Neurosci. 30, 8214–8220 (2010).
Google Scholar
Wu, Y. et al. Calmodulin kinase II is required for fight or flight sinoatrial node physiology. Proc. Natl Acad. Sci. USA 106, 5972–5977 (2009).
Google Scholar
Temkin, P. et al. The retromer supports AMPA receptor trafficking during LTP. Neuron 94, 74–82.e5 (2017).
Google Scholar
Uphoff, C. C. & Drexler, H. G. in Cancer Cell Culture: Methods and Protocols 2nd edn (ed. Cree, I. A.) Ch. 8 (Humana, 2011).
Aoto, J., Martinelli, D. C., Malenka, R. C., Tabuchi, K. & Südhof, T. C. Presynaptic neurexin-3 alternative splicing trans-synaptically controls postsynaptic AMPA receptor trafficking. Cell 154, 75 (2013).
Google Scholar
Lloyd, B. A., Han, Y., Roth, R., Zhang, B. & Aoto, J. Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus. Nat. Commun. 14, 4706 (2023).
Google Scholar
Yang, Y. et al. Endophilin A1 regulates dendritic spine morphogenesis and stability through interaction with p140Cap. Cell Res. 25, 496–516 (2015).
Google Scholar
Restrepo, S., Langer, N. J., Nelson, K. A. & Aoto, J. Modeling a neurexin-3α human mutation in mouse neurons identifies a novel role in the regulation of transsynaptic signaling and neurotransmitter release at excitatory synapses. J. Neurosci. 39, 9065–9082 (2019).
Google Scholar
Zhu, B., Eom, J. & Hunt, R. F. Transplanted interneurons improve memory precision after traumatic brain injury. Nat. Commun. 10, 5156 (2019).
Google Scholar
Chen, Y. C., Mao, H., Yang, K. H., Abel, T. & Meaney, D. F. A modified controlled cortical impact technique to model mild traumatic brain injury mechanics in mice. Front. Neurol. 5, 100 (2014).
Google Scholar
Purkey, A. M. et al. AKAP150 palmitoylation regulates synaptic incorporation of Ca2+-permeable AMPA receptors to control LTP. Cell Rep. 25, 974–987.e4 (2018).
Google Scholar
Paul, G., Cardinale, J. & Sbalzarini, I. F. Coupling image restoration and segmentation: a generalized linear model/bregman perspective. Int. J. Comput. Vis. 104, 69–93 (2013).
Google Scholar
Rizk, A. et al. Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh. Nat. Protoc. 9, 586–596 (2014).
Google Scholar
Frankowski, J. C. et al. Brain-wide reconstruction of inhibitory circuits after traumatic brain injury. Nat. Commun. 13, 3417 (2022).
Google Scholar
Park, J. S. et al. Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal. Proc. Natl Acad. Sci. USA 111, 5896–5901 (2014).
Google Scholar
Stachniak, T. J., Ghosh, A. & Sternson, S. M. Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus→midbrain pathway for feeding behavior. Neuron 82, 797–808 (2014).
Google Scholar
Derdeyn, P. Quantifying number and density of neural puncta. Zenodo https://doi.org/10.5281/zenodo.10199183 (2023).