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Mass testing of mutant enzymes involved in photosynthesis

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Nature, Published online: 22 January 2025; doi:10.1038/d41586-025-00104-4

Rubisco is the enzyme responsible for carbon fixation in plants, algae and many bacteria. A screen of a comprehensive library of rubisco variants (each with a different single-amino-acid mutation), expressed in engineered bacteria, identifies several mutations that improve the enzyme’s affinity for carbon dioxide.

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